Presenters James Burtis of Cornell University and John Shepard of the Connecticut Agricultural Experiment Station provide an overview of practical guidance for conducting pesticide resistance bioassays for mosquitoes in the Northeast US. Topics covered include: What is pesticide resistance and how does it emerge? How are resistance bioassay diagnostics created? How do you determine the resistance status of field-collected mosquitoes? How can the results of these resistance tests inform management? What are the best methods and life stages to collect from the field for resistance assays? How do you rear different mosquito species in the laboratory for resistance assays? and Reporting data to MosquitoNET.