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A bulked segregant analysis of pyrethroid resistance in Musca domestica

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dc.contributor.authorFreeman, Jamie Catherine
dc.contributor.chairScott, Jeffrey G.
dc.contributor.committeeMemberWang, Ping
dc.contributor.committeeMemberMesser, Philipp
dc.date.accessioned2021-03-15T13:32:25Z
dc.date.available2022-01-11T07:00:28Z
dc.date.issued2020-12
dc.description105 pages
dc.description.abstractIncreased detoxification of insecticide is a common method of insecticide resistance. Despite this commonality, the molecular changes that are responsible are for are generally poorly understood. Often little is known beyond the involvement of particular enzyme families in the resistance phenotype or some number of overexpressed detoxification genes that may be involved. The availability of forward genetic studies in pest species will prove invaluable to advancing our understanding of resistance and insecticide toxicology in general. We use a quantitative trait mapping method called bulk segregant analysis (BSA) to determine the basis of a multigenic pyrethroid resistance phenotype to the known target-site of pyrethroids, the voltage-gated sodium channel, and a cluster of cytochrome P450s (CYPs) on chromosome 5. Structural variant calling using long read sequences indicates the area linked to resistance on chromosome 5 is structurally complicated in the resistant strain, and RNA-seq data indicates overexpression of 8 CYPs at this locus. The BSA mapping results are supported by linkage mapping of resistance with known morphological makers and synergist bioassays that indicate involvement of esterase and CYP-mediated detoxification in the resistance phenotype.
dc.identifier.doihttps://doi.org/10.7298/xscv-7y87
dc.identifier.otherFreeman_cornell_0058O_11112
dc.identifier.otherhttp://dissertations.umi.com/cornell:11112
dc.identifier.urihttps://hdl.handle.net/1813/103246
dc.language.isoen
dc.subjectbulked segregant analysis
dc.subjectinsecticide resistance
dc.subjectMusca domestica
dc.subjectpyrethroid
dc.titleA bulked segregant analysis of pyrethroid resistance in Musca domestica
dc.typedissertation or thesis
dcterms.licensehttps://hdl.handle.net/1813/59810
thesis.degree.disciplineEntomology
thesis.degree.grantorCornell University
thesis.degree.levelMaster of Science
thesis.degree.nameM.S., Entomology

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