Library

eCommons

 

SINGLE-MOLECULE STUDIES ON EUKARYOTIC TOPOISOMERASE II AND MECHANICAL PROPERTIES OF EUKARYOTIC CHROMATIN

Simple item page
dc.contributor.authorPark, Seong ha
dc.contributor.chairWang, Michelle D.
dc.contributor.committeeMemberSmolka, Marcus B.
dc.contributor.committeeMemberChen, Peng
dc.date.accessioned2022-09-15T15:51:18Z
dc.date.issued2022-05
dc.description183 pages
dc.description.abstractDNA replication inevitably encounters topological impasses created from the double helical structure of double stranded DNA. Incomplete resolution of the supercoils generated during fork progression leads to intertwining, or catenation, of the two sister chromatids which in turn leads to deleterious downstream effects such as aneuploidy. While biochemical aspects of regulation of catenation prevention and topoisomerase II, the enzyme primarily responsible for resolving supercoils during replication, activity on naked DNA have been deeply explored, the role of mechanical properties of chromatin and topoisomerase II activity on eukaryotic chromatin in its highly compacted form has been largely overlooked. Here I present single-molecule studies that provide insight into the effect of chromatin compaction via formation of nucleosomes in eukaryotic DNA replication and topoisomerase activity. First is the characterization of torsional mechanics of eukaryotic chromatin and its consequences followed up by observation of topoisomerase II activity on nucleosomal DNA. Secondly, I suggest a methodology to dissect the kinetic details of topoisomerase II activity and use it to observe response of the enzyme in physiologically relevant magnesium concentrations.
dc.identifier.doihttps://doi.org/10.7298/1ha9-by62
dc.identifier.otherPark_cornellgrad_0058F_12923
dc.identifier.otherhttp://dissertations.umi.com/cornellgrad:12923
dc.identifier.urihttps://hdl.handle.net/1813/111771
dc.language.isoen
dc.rightsAttribution-ShareAlike 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-sa/4.0/
dc.subjecteukaryotic chromatin
dc.subjecteukaryotic DNA replication
dc.subjectmagnetic tweezers
dc.subjectoptical tweezers
dc.subjectsingle-molecule
dc.subjecttopoisomerase II
dc.titleSINGLE-MOLECULE STUDIES ON EUKARYOTIC TOPOISOMERASE II AND MECHANICAL PROPERTIES OF EUKARYOTIC CHROMATIN
dc.typedissertation or thesis
dcterms.licensehttps://hdl.handle.net/1813/59810.2
thesis.degree.disciplineBiophysics
thesis.degree.grantorCornell University
thesis.degree.levelDoctor of Philosophy
thesis.degree.namePh. D., Biophysics

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Park_cornellgrad_0058F_12923.pdf
Size:
4.79 MB
Format:
Adobe Portable Document Format
Download

Collections

Cornell Theses and Dissertations