Data from: The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract

Hafezi, Yassi; Omurzakov, Arsen; Carlisle, Jolie A.; Caldas, Ian V.; Wolfner, Mariana F.; Clark, Andrew G.

Data from: The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract

dc.contributor.author	Hafezi, Yassi
dc.contributor.author	Omurzakov, Arsen
dc.contributor.author	Carlisle, Jolie A.
dc.contributor.author	Caldas, Ian V.
dc.contributor.author	Wolfner, Mariana F.
dc.contributor.author	Clark, Andrew G.
dc.date.accessioned	2023-07-05T14:24:15Z
dc.date.available	2023-07-05T14:24:15Z
dc.date.issued	2023-06-30
dc.description	Suggested citation: Hafezi, Y., Omurzakov, Arsen, Carlisle, J., Vasconcellos Caldas, I., Wolfner, M., & Clark, A. (2023). Data from: The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract [Dataset]. Cornell University Library eCommons Repository. https://doi.org/10.7298/VTR8-AB60
dc.description	CHANGELOG: Added two additional file bundles on 2023-09-21: Hafezietal2023_WDYCodemlAnalysisFiles.zip Hafezietal2023_WDYFinalAnnotations.zip	EN
dc.description	CHANGELOG: added .csv to Sperm Head Swimming Videos tar file, updated readme.
dc.description.abstract	These files contain data supporting the results reported in Hafezi et al, 2023, 'The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract.' In Hafezi et al, 2023 we created CRISPR mutants in Drosophila of the Y-linked gene, WDY, which is required for male fertility. WDY mutants produced mature sperm with beating tails that could be transferred to females but failed to enter the female sperm storage organs. We demonstrated that WDY mutant sperm’s tails beat approximately half as fast as wild-type sperm and that sperm do not propel themselves within the male ejaculatory duct or female reproductive tract (RT). These specific motility defects likely caused the sperm storage defect and sterility of the mutants. Regional and genotype-dependent differences in sperm motility suggested that sperm tail beating and propulsion do not always correlate. Furthermore, we found significant differences in the hydrophobicity of key residues of a putative calcium-binding domain between orthologs of WDY that are Y-linked and those that are autosomal. WDY was previously shown to be under positive selection. Together these findings suggest that WDY is undergoing functional evolution that coincides with its transition from autosomal to Y-linked in Drosophila melanogaster and its most closely related species. Finally, we showed that mutants for another Y-linked gene, PRY, also show a sperm storage defect that may explain their subfertility. In contrast to WDY, PRY mutants did swim in the female RT, suggesting they are defective in yet another mode of motility, navigation, or a necessary interaction with the female RT. Overall, we provide direct evidence for the long-held presumption that protein-coding genes on the Drosophila Y regulate sperm motility.
dc.identifier.doi	https://doi.org/10.7298/vtr8-ab60
dc.identifier.uri	https://hdl.handle.net/1813/113323
dc.relation.isreferencedby	Yassi Hafezi, Arsen Omurzakov, Jolie A. Carlisle, Ian V. Caldas, Mariana F. Wolfner, Andrew G. Clark. (2024) The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract. Commun Biol 7, 90. https://doi.org/10.1038/s42003-023-05717-x
dc.relation.isreferencedbyuri	https://doi.org/10.1038/s42003-023-05717-x
dc.rights	Attribution 4.0 International
dc.rights.uri	https://creativecommons.org/licenses/by/4.0/
dc.subject	sperm motility
dc.subject	Y-chromosome
dc.subject	Drosophila
dc.subject	evolution
dc.title	Data from: The Drosophila melanogaster Y-linked gene, WDY, is required for sperm to swim in the female reproductive tract
dc.type	dataset