Hemp (Cannabis sativa L.) is primarily grown for the medicinally beneficial secondary metabolites produced by pistillate inflorescences. Micropropagation is a valuable method of propagating hemp plants due to the aseptic process and the production of true-to-type propagules that are generated in minimal space. The cultivar ‘TJ’s CBD’ was utilized for a series of experiments to test media inputs and practices for the purpose of clonal micropropagation of hemp. Stage I stock plants that were grown in a growth chamber produced less contamination in newly established cultures than plants that were grown in a greenhouse. Stage II culture factors such as sucrose concentration, media type, temperature, and plant growth regulator type and concentration dramatically influence growth in vitro. The pH, explant type, and gelling agent type had less influence on plant growth. Stage III in vitro rooting can be bypassed and plants can be rooted ex vitro during Stage IV acclimation with a dome and additional auxin treatments.