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Engineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences

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dc.contributor.authorChen, Feng-Yang
dc.contributor.chairDelisa, Matthew
dc.contributor.committeeMemberDaniel, Susan
dc.contributor.committeeMemberPaszek, Matthew J.
dc.date.accessioned2019-10-15T16:49:27Z
dc.date.available2019-10-15T16:49:27Z
dc.date.issued2019-08-30
dc.description.abstractThe key enzyme in the Campylobacter jejuni glycosylation pathway, PglB, has been one of the well-studied asparagine-linked oligosaccharyltransferase (OST) in bacterial glycoengineering area. While C. jejuni PglB performs well in transferring different glycans, the acceptor sites it can recognize are limited. We provided a thorough investigation into Desulfovibrio desulfuricans PglB (DdPglB), an OST sharing homology with C. jejuni PglB but having more relaxed sequon specificity. The sequon logo of DdPglB against XXNXT library were built through a high-throughput assay. Two engineered OSTs combining distinct transmembrane domain and periplasmic domain of PglBs from C. jejuni and D. desulfuricans was also investigated. And the study interestingly revealed they can lose all their glycosylation activities after the combination. According to the sequon logo we built and further verification, a notable sequon QYNST, which is a native acceptor sequon of Fc domain of human immunoglobulin G, was able to be glycosylated by DdPglB. Overall, we presented a series of sequons that can be glycosylated by DdPglB and further expanded the glycoengineering toolbox with more potential acceptor sites that were not glycosylated before.
dc.identifier.doihttps://doi.org/10.7298/s792-kt31
dc.identifier.otherChen_cornell_0058O_10624
dc.identifier.otherhttp://dissertations.umi.com/cornell:10624
dc.identifier.otherbibid: 11050647
dc.identifier.urihttps://hdl.handle.net/1813/67663
dc.language.isoen_US
dc.subjectChemical engineering
dc.subjectCampylobacter jejuni
dc.subjectDesulfovibrio desulfuricans
dc.subjectGlycosylation
dc.subjectOligosaccharyltransferases
dc.subjectPglB
dc.subjectsequon logo
dc.subjectBiomedical engineering
dc.titleEngineered Oligosaccharyltransferases With Unique N-Glycosylation Site Preferences
dc.typedissertation or thesis
dcterms.licensehttps://hdl.handle.net/1813/59810
thesis.degree.disciplineChemical Engineering
thesis.degree.grantorCornell University
thesis.degree.levelMaster of Science
thesis.degree.nameM.S., Chemical Engineering

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