CHEMICAL STRUCTURE ELUCIDATION OF AN UNKNOWN COMPOUND BY DIRECT ANALYSIS IN REAL-TIME MASS SPECTROMETRY AND NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
Direct Analysis in Real-Time Mass Spectrometry (DART-MS) and Nuclear Magnetic Resonance (NMR) are two analytical techniques that are most used in structural chemistry and biology. DART-MS measures mass to charge ratio of different compounds in a solution. From the mass to charge ratio, the chemical formula of a substance can be deduced. NMR can determine the chemical structure of a purified substance. Thus, with the chemical information of molecules gathered by DART-MS and NMR, chemical structures of the molecules can be unambiguously determined. In this thesis, the chemical structure of an unknown compound was found by DART-MS and NMR. The unknown compound was dissolved in chloroform (CDCl3) solvent, and the exact mass of the unknown compound was measured by DART-MS. After the mass measurement, the solution was run in NMR for the 1H NMR and 2D NMR. With the data from DART-MS and NMR structure, the chemical structure of the compound was determined to be nuciferine.
Zax, David B.
Davis, Harry Floyd
Chemistry and Chemical Biology
M.S., Chemistry and Chemical Biology
Master of Science
dissertation or thesis