MICROPROPAGATION AND IN VITRO MUTAGENESIS OF VITEX AGNUS-CASTUS
Nor Hisham Shah, Nor Kamal Ariff
ABSTRACT Vitex agnus-castus (Linnaeus, C., 1799) has many ornamental properties that make it a popular ornamental plant. Currently however, the shrub is overlooked and underutilized because of a few weaknesses. These weaknesses could be eliminated by creating polyploid versions of the plant using in vitro chemical mutagenesis. To achieve this, the micropropagation protocol for V. agnus-castus has been outlined and the effects of oryzalin exposure to the plant in vitro recorded. For micropropagation, cuttings were surfaced sterilized using detergent and bleach solution and were successfully introduced into sterile medium with 94% success rate. To multiply the cuttings, two cytokinins were tested at various concentrations. It was observed that hormone 6-benzylaminopurine (BA) at concentration of 4.44 µM is the most effective at inducing shoot formation without compromising the length of shoots produced as compared to hormone 6-(γ,γ-dimethylallylamino)purine (2iP). To induce root formation, two auxins were tested at various concentrations and the hormone indole-3-butyric acid (IBA) at concentration 5 µM was observed to be the most effective when compared to 1-naphthaleneacetic acid (NAA) after eight weeks of growth. Lastly, rooted tissue culture plants were successfully acclimated into the greenhouse with 85% survival during the acclimatization protocol. The application of oryzalin to in vitro plants produced phenotypic changes and the percent survival of V. agnus-castus after exposure to oryzalin was recorded at different concentrations and exposure times. After 16 weeks of observation, the lethal dose with 50% survival rate to oryzalin was determined to be at a concentration of 0.3 milimolar for 60 minutes exposure time.
Cellular biology; Horticulture; in vitro; Micropropagation; Mutagenesis; Tissue culture; Vitex agnus-castus; Plant sciences
Bridgen, Mark P.
Bassuk, Nina Lauren
M.S., Plant Breeding
Master of Science
dissertation or thesis