Bmac And Prp Enhance Migration Of Endogenous Mscs
Bone marrow derived mesenchymal stem cells (MSCs) demonstrate promise for musculoskeletal regenerative medicine. An alternate approach to the direct delivery of stem cells is to exploit the concept of in situ tissue engineering. Biologics such as bone marrow aspirate concentrate and platelet rich plasma (PRP) enhance recovery from musculoskeletal injuries. These biologics contain growth factors which could act as chemoattractants for stem cells. In this project, we observed the migratory capacity of stem cells in response to biologics using of a microfluidics device. The purpose of this study was to identify the optimal biologic for recruitment of stem cells. Our hypothesis was that PRP would result in the greatest migration of MSC's because of the milieu and concentration of growth factors contained in PRP. We found that all biologics tested resulted in an increase in migration of stem cells compared to the control. This demonstrates that biologics can be used as chemotactic agents to recruit MSCs to a site of injury. This information will reduce the need and therefore the risks and costs associated with direct stem cell delivery.
mesenchymal stem cells; bone marrow aspirate concentrate; platelet rich plasma
Fortier, Lisa Ann
Lin, David M.; Gilbert, Robert Owen
M.S. of Veterinary Medicine
Master of Science
dissertation or thesis