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Melatonin in Tart Cherries: Methods of Extraction and Detection

Author
Lang, Megan Joy
Abstract
Melatonin, a powerful antioxidant, offers potential human benefits in the fields
of medicine, nutrition, and food science. While best understood in a
mammalian system, melatonin has been identified in plants and dietary
melatonin has been shown to increase circulating levels in the blood. Thus,
there exists a great interest in extracting and detecting melatonin present in
edible plant matrices. Extraction techniques such as liquid/liquid, solid phase,
and solid/liquid extraction were investigated and compared to determine the
best approach for isolating melatonin from fruit. Enzyme-linked immunoassay
(ELISA), fluorescence, and mass spectrometry were investigated for their use
as detection methods for melatonin originating in fruit systems. Additionally,
the stability of melatonin in a pH 3.5 buffered model system was studied to
gain preliminary information regarding melatonin heat and light stability. It was
determined that melatonin is both heat stable and light stable for up to one
hour (85C and 17 par, investigated separately). Solid/liquid extraction using
ethyl acetate as a solvent was determined to be the best extraction procedure
while high performance liquid chromatography-mass spectrometry with the
use of a deuterated internal standard was the preferred detection method. A
significant amount of work remains in the area of quantification of melatonin
from fruit systems.
Description
Olga I. Padilla-Zakour, Mary H. Tabacchi
Date Issued
2008-07-30Subject
Melatonin; Cherry; Cherries; Montmorency
Type
dissertation or thesis