STRUCTURAL AND FUNCTIONAL CHARACTERIZATION OF A MULTI-DOMAIN NITRIC OXIDE SYNTHASE AND A BACTERIAL SWARMING EFFECTOR, SWRD
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Nitric Oxide (NO) is a potent free radical that is enzymatically produced by NO synthases (NOS) through complex electron transfer mechanisms that leverage multiple cofactors. NOSs have eluded full-length structural characterization. I will discuss the structural characterization of a multidomain NOS found in Synechococcus PCC 7335, that revealed unique flavin-reduction chemistry and a built-in Ca2+-dependent regulatory domain. Bacterial swarming is an emergent property that allows cells to rapidly colonize advantageous environments. When bacteria swarm, they require higher-torque generation that is facilitated through modification of their flagellar motors. In this context, we will explore the structure of SwrD, a small protein that influences torque generation in B. subtilis. We leverage high-throughput in silico pulldowns, genetic knock-out studies and x-ray crystallography to implicate SwrD in direct interaction with the flagellar motor and regulation of flagellar gene expression.