The commercial production of strawberries faces many challenges to farm profitability and viability, including fumigation bans, plant availability, and the spread of soil-borne pathogens. Field propagated bare-root plants (Fbrp) are the most commonly used planting material, but the production and use of Fbrp has been criticized for being unsustainable, unable to meet grower demands, and a common way for soil-borne pathogens to enter fields. As a potential solution, controlled environment-based propagation systems have been proposed as an alternative to field-based propagation systems, but there is little research on how this novel strawberry plant propagation method may affect plant phenotype, yield, and fruit quality. This thesis investigates the differences in plant performance between the industry standard and the novel propagation method. Strawberry cultivars Albion, Cabrillo, and Monterey were propagated either via standard field procedure as bare-root plantlets or via controlled environment as plug plants and were subsequently measured through a regionally standard field trial to compare potential phenotypic differences surrounding vegetative, harvest, and fruit chemical properties. Results indicated that Controlled Environment Agriculture propagated plug plants (CEApp) were overall more vigorous than Fbrp. During the vegetative analysis, CEApp were found to have significantly higher quantities of branch crowns and overall higher plant dry mass. In the harvest analysis, CEApp also had higher quantities of fruit, overall mass of fruits, and average individual fruit mass. There were no significant differences between CEApp and Fbrp in the quantity of runners produced, average largest fruit, brix (ºBx), titratable acidity (TA), ºBx:TA ratio, or pH. This research has provided validation that the novel system is a viable alternative to field-based propagation systems. Growers can opt for CEApp to decrease the risk of soil-borne pathogens entering their production systems and increase planting date flexibility, while also increasing their yield and fruit quality.