Given the rapid growth of the industrial Cannabis sector, the necessity for a reliable source of starter plant with limited genetic variation and efficient growth is crucial to achieving reliable and successful cultivation results. This study presents a multi-faceted experiment series analyzing propagation techniques for evaluating proficiency in growth and development of Cannabis plants. The research encompasses various (1) soilless propagation methods ((i) aeroponics, (ii) horticultural foam, and (iii) rockwool) and (2) transplant timings, (3) aeroponic spray intervals, and (4) aeroponic reservoir nutrient concentrations to elucidate their impact on rooting and growth parameters amongst two cultivars. It was found that aeroponics can provide as or more effective root development and plant growth than soilless propagation substrates. Further, continuous spray intervals compared to intermittent and optimized nutrient concentrations result in better promotion of root initiation and plant growth in aeroponics. The effects of experimental treatment often depended on cultivar and sampling day. Cultivators should assess their specific genetics to pinpoint the optimal conditions for propagation. These findings offer valuable insights into how various propagation techniques and growth parameters can be tailored to enhance the cultivation process. These results hold critical implications for cultivators intending to achieve premium harvests through efficient propagule methods and optimization strategies in the competitive Cannabis industry.