Pancytopenia is defined as a combination of anemia, leukopenia and thrombocytopenia. The causes of pancytopenia include a variety of infectious, drug- and toxin-induced, immune-mediated, and neoplastic conditions. The most important factor in resolving pancytopenia is to determine the primary cause. Determining the underlying cause of pancytopenia will help to establish the correct treatment protocol and determine the prognosis of the patient. This report will discuss a presumptive case of immune-mediated pancytopenia in an eight year old female infact Rottweiler, along with the clinical findings, potential diagnoses, diagnostics, available treatments, and outcome.