A 2 year old female miniature donkey with severe disseminated Besnoitiosis presented for necropsy. The donkey had an 8 month history of progressive skin lesions characterized by crusting, alopecia, hyperpigmentation, and thickened skin with cachexia and subsequent laminitis. An antemortem diagnosis of Besnoitiosis was made via skin biopsy submitted by the referring veterinarian. Euthanasia was elected due to poor response to therapy and a poor prognosis. The animal was donated for necropsy to better characterize the extent and nature of her disease. This is the most recent and third case of disseminated Besnoitiosis in an equid within the United States.