In August of 2012, three adult, female, roughtail stingrays, (Dasyatis centroura), were found in an intake pool at a power plant in central Florida. They had become accidentally entrapped and were subsequently retrieved for display in a large public aquarium. Aside from superficial abrasions and tail wounds all three rays, (RTR1, RTR2, and RTR3), appeared to be in good physical condition. Blood was successfully collected from two of the rays for baseline comparison, and the rays were transported to a satellite facility to undergo quarantine. All three animals received antibiotics, topical wound management as needed and prophylactic treatment for parasitic infection. After a 65 day quarantine period, the rays were transferred to the main aquarium facility and acclimated in a secondary system for several days before being released into a 6.3 million gallon, multi-species exhibit. Seven months later one of the rays, (RTR3), was found dead in the exhibit. This paper will discuss the necropsy findings and cause of death for RTR3. In addition, it will describe diagnostics performed and subsequent treatment of the remaining two roughtail rays.