The Regional Wildlife Disease Positives Software allows the user to track the occurrence, persistence, or spread of chronic wasting disease (CWD) in free ranging white-tailed deer (Odocoileus virginianus) in select states and provinces in the eastern United States (US) and Canada (CAN). The Regional Wildlife Disease Positives Software was adapted from the Wildlife Disease Positives Software (Mitchell et al. 2021) to display pooled data and results up to the continental scale. The R scripts of the Regional Wildlife Disease Positives Software contain a sequential network of commands that format the geospatial files and surveillance (testing) data, then launch the user interface (UI) of the interactive software to depict pooled results at the continental scale. The Regional Wildlife Disease Positives Software packet includes (redacted) disease testing data from a select assortment of United States wildlife agencies: Alabama, Arkansas, Connecticut, Florida, Georgia, Indiana, Iowa, Kentucky, Louisiana, Maryland, Michigan, Minnesota, Mississippi, New Hampshire, New York, North Carolina, Ohio, Pennsylvania, Rhode Island, Tennessee, Virginia, and Wisconsin and Canadian provincial agencies: Ontario. While the Regional Wildlife Disease Positives Software was designed for use by state and provincial agencies to track chronic wasting disease (CWD) in white-tailed deer from 2013-2025, the software is easily adaptable to other wildlife diseases in other states or provinces, in different years, and/or in differing wildlife species.