Bioprinting, a form of additive manufacturing, uses cell-laden hydrogels to produce highly customizable constructs for applications in tissue engineering and regenerative medicine. Despite its potential, several challenges continue to impede the clinical translation of bioprinted constructs, particularly regarding reproducibility and quality assurance. Current quality control practices rely on the evaluation of critical quality attributes (CQAs) at various stages of the bioprinting workflow. These assessments are predominantly performed offline using destructive analytical techniques, which necessitate the fabrication of multiple constructs. However, reproducing identical constructs remains problematic due to the variability in bioink properties, which are time-sensitive and require extensive optimization, as well as the complex and variable nature of cell collection and processing procedures. Additionally, inconsistencies between printed constructs further complicate quality control efforts. As an alternative, the integration of biosensors directly into the bioprinting process presents a promising strategy for enabling real-time, non-destructive monitoring of CQAs, thereby enhancing process control and construct reproducibility. The application of real-time monitoring technologies in bioprinting, along with their potential uses and future directions, is explored in Chapter 1. This evaluation of the current state of the field highlighted a significant gap: the limited development of real-time, label-free methods for monitoring cellular behavior during the bioprinting process. Such monitoring is essential for ensuring the consistency and clinical viability of bioprinted constructs. In response to this need, Chapter 2 demonstrates the feasibility of using dielectric impedance spectroscopy as an in-line method for assessing cell concentration and viability directly from a syringe. To further assess the utility of this approach within a bioprinting context, Chapter 3 examines how variations in alginate bioink properties affect the real-time detection of cells. Finally, to expand upon the applications for this technology, Chapter 4 presents modifications to the electrode housing and geometry, which resulted in improved manufacturability and heightened sensitivity to a wide range of cell concentrations.