Marine biofouling is the one of the biggest problems for ships sailing in lakes and oceans around the world. The drag from various organisms adhering to a ship’s hull, like mussels, diatoms, algae, mollusks, and the like reduce the efficiency of ships moving through the water and result in enormous financial and fuel losses. The development of the field of antifouling and fouling release coatings over the years has been instrumental in preventing the growth of such organisms on the ships. One of the major polymers used for antifouling coating purposes is poly(dimethyl siloxane) (PDMS) which is hydrophobic in nature. But PDMS alone is not sufficient in reducing the growth of biofouling on the ships and hence, in recent years, modification of PDMS with various functional groups has been studied to give both antifouling as well as fouling release properties to the coating. Here, a siloxane block copolymer, PS-PVMS, which has both a hydrophobic and rigid PS part, and a modifiable, flexible PVMS part, is functionalized with various compounds containing fluorinated groups, PEG groups and zwitterions, via a thiol-ene click reaction to obtain a modified polymer. The resulting modified PS-PVMS is then coated on glass slides or silicon wafers by using different methods like spin coating and spray coating, and the coated substrates are characterized by WCA measurements, bubble contact angle goniometry, AFM and XPS to determine their hydrophilic or hydrophobic nature. Antifouling tests are performed on the coated substrates under laboratory conditions using biofouling assays to check their behavior against various fouling species. This helps in determining their real-life efficacy and guides the future development of antifouling and fouling-release coatings.