The issue of sedentary behavior in the workplace and its negative effects on health have stirred discussions around implementing a more proactive approach to promote physical movement, particularly in the realms of design and the built environment. The present study looks at the attributes of spatial design that play a role in promoting physical movement in the workplace, specifically in encouraging walking behaviors and stand-up breaks throughout the day. This study utilizes multi-method research framework to compare individual physical activity habits, characteristics of the built environment, physical design attributes, and user behaviors. The outcomes of this study observed a relationship between the design of built environment and users’ response and their associated physical movement in the workplace.