This study aims to explore the Missouri wine industry prior to Prohibition through the creation of a historical narrative, an analysis of the most popular wine grape varieties of the time, and a genetic analysis of remnant pre-Prohibition grapevines in the region. At one time, Missouri was an important and leading wine producer for the United States, and its story has not been fully described, therefore this study explores it in depth. Collecting pre-Prohibition grapevine samples throughout Missouri, these samples will undergo genetic marker analysis and comparison to the Cornell database produced as part of the VitisGen 1 and VitisGen2. While the genetic pedigree analysis is ongoing, the goal is to identify mystery vines and create a network of relational mapping between the collected grapes which may ultimately impact modern grape breeding efforts. Future research may allow for the return of some of these lost pre-Prohibition varieties.