This thesis investigates strategies to enhance a novel maize transformation approach, resulting in improved transformation responses across multiple maize genotypes. The method, known as Agrobacterium-mediated leaf whorl transformation and developed by Corteva Agriscience, represents an advancement over traditional techniques. However, its optimization remains largely unexplored. This work evaluates modifications to the protocol at multiple stages and assesses their effects across several genotypes.Modifications to all stages of the method were investigated. Extending the co-cultivation period and supplementing the co-cultivation medium with antioxidants, including DTT and L-cysteine, improved transformation responses in select genotypes. Reduced concentrations of the selection agent G418, the use of Gelzan as a gelling agent, and implementation of a gradual light exposure environment during shoot regeneration further enhanced genotype response. These findings offer a foundation for researchers seeking to apply this technology and represent, to our knowledge, the first comprehensive report of optimization strategies for this system.