Adenosine triphosphate (ATP) is a universal source of energy for all living organisms. It plays a fundamental intracellular and extracellular role in regulation of cellular metabolism, cell signal pathways and biochemical reaction. Its fundamental research and diverging application developed really fast, especially after the discovery of ATP aptamer. With a great development, the ATP aptamer has been applied to a wide range of area, such as biosensor, biomarker and some other detection. In view of the flexibility and versatile sequence and structure of nucleic acid, aptamer technology can be used at almost everywhere and its advantage is also striking, in lots of aspects even better than the antibody. At the same time, rolling circle amplification is in great booming, which can be utilized to rapidly copy multiple of DNA sequence by a circular DNA. Its repeated nucleic acid sequence, provide a really good vector for ATP aptamer in application. In my experiment, we produce a RCA-dependent ATP aptamer-incorporated DNA hydrogel to measure the amount of ATP that can be captured by DNA hydrogel.