This study proposed different materials of microfluidic mixer fabrication and their flow test. Two different design based on a T-mixer were fabricated. J-shaped and vertical pillar micromixers were fabricated with Zeonor, PDMS, silicon and SU-8. Several methods were used to seal mixer and test the sealing effect. Using Cy5 and fluorescein to measure the mixing time of micromixer. The mixing results shows that the J-shaped micromixer has a good mixing function and a short mixing time.