Abandoned oil and gas wells are significant sources of methane emissions to the atmosphere. There are millions of abandoned wells in the United States, so there is a critical need for low-cost and sustainable approaches for mitigating methane emissions from these sources. In this study, we designed and tested methanotrophic biofilters as passive tools to mitigate methane emissions from abandoned wells. We first assessed the biokinetics of methane oxidation in batch tests with enrichments from wetland sediments as well as biofilms from membrane bioreactors with Methylosinus trichosporium OB3b, Methycystis parvus OBBP and Methylomicrobium album BG8 and two Methylomonas sp. at a range of temperatures spanning seasonal variation in air temperature in the Appalachian region, where a high density of abandoned wells are located. Cultures with the highest maximum reaction velocities (Vmax) were then tested in laboratory-scale flow-through bio-trickling filter configurations with packing materials including gravel, sponge, and biofilm carriers. Batch and column studies were used to parameterize biokinetic models to describe methane removal efficiencies in flow-through systems under field-relevant temperatures, flow rates, and methane concentrations. Modeling indicates that a 1 m_ sponge-packed biofilter can achieve from 19% to 34% methane removal at 22°C, and from 4% to 7% at 5°C in the Appalachian region assuming first order kinetics.