Data in support of research: Pseudomonas introduced after pasteurization during the processing and packaging of fluid milk is detrimental to product quality over refrigerated shelf-life. At present, there are limited options available to dairy processors to identify, diagnose, and track Pseudomonas in products and processing environments. We evaluated 7 potential genes to ultimately describe a single-gene PCR and sequencing process for rapid and low-resource preliminary subtyping and identification of dairy spoilage-associated Pseudomonas. Application of this process by dairy processors will allow for more effective root cause analysis of spoilage events and ongoing quality concerns, ultimately resulting in improved milk quality and shelf-life.