Healthy adult females of East Asian (EA) ancestry have been reported to have higher iron (Fe) stores and Fe absorption and alterations in Fe regulatory hormones when compared to adult females of Northern European (NE) ancestry. This cross-sectional study collected data on menstrual characteristics among adult females of genetically confirmed EA or NE ancestry and evaluated the potential impact of this factor on Fe status. Data were obtained from premenopausal females aged 18-50 years (BMI 18–30 kg/m²) who were enrolled in the FeGenes study, which recruited healthy individuals of genetically confirmed EA or NE ancestry. Data on age at menarche, cycle frequency, menstrual bleeding duration, and average use of menstrual products were collected from self-reported questionnaires, and menstrual blood loss (MBL) scores were calculated. Fe status was assessed through serum ferritin (SF; µg/L), hepcidin (ng/mL), soluble transferrin receptor (sTfR; mg/L), transferrin (TF; mg/dL), and calculated total body iron (TBI; mg/kg). Group comparisons and associations with Fe status biomarkers were examined. EA females had an earlier median age at menarche (EA 12.0 [IQR 11.0, 13.0] years; NE 13.0 [IQR 12.0, 13.3] years; p = 0.002) and a higher MBL score (EA 24.0 [IQR 17.5, 31.5]; NE 16.0 [IQR 10.5, 27.9]; p < 0.001) than NE females. In NE individuals, a higher MBL score was significantly associated with lower log-transformed SF (β = -0.01; SE = 0.005; p = 0.004), TBI (β = -0.06; SE = 0.02; p = 0.002), and higher log-transformed sTfR (β = 0.005; SE = 0.001; p = 0.002). No associations between MBL score and Fe status were evident in EA females. In conclusion, higher MBL scores were associated with lower Fe status in NE but not EA females. Greater attention and validated metrics are needed to evaluate menstrual blood losses and better quantify Fe needs in reproductive-aged women.