Iron deficiency, the most prevalent micronutrient deficiency globally, affects over two billion people and remains a pressing public health challenge. As the primary cause of anemia, it underscores the need for more effective dietary strategies that enhance iron bioavailability. While conventional food fortification has improved iron content in staple crops, it typically yields low iron absorption, undesirable sensory changes, and gastrointestinal discomfort. To address these limitations, this study explores a biofortification approach using whole wheat engineered to overexpress nicotianamine-- a natural iron and zinc chelator present in higher plants. Using the Gallus gallus (chicken) model and intraamniotic administration, we investigated the effect of nicotianamine-chelated iron on gastrointestinal function and systematic iron status. The findings show the potential of nicotianamine-based biofortification to improve micronutrient status and address the global burden of hidden hunger.