These files contain data supporting all results reported in Nihranz et al. "Adverse effects of inbreeding on the transgenerational expression of herbivore-induced defense traits in Solanum carolinense". In Nihranz et al. we found: We found that feeding by Manduca sexta caterpillars on maternal Solanum carolinense plants had a positive influence on trichome and spine production in offspring and that caterpillar development on offspring of herbivore- damaged maternal plants was delayed relative to that on offspring of undamaged plants. Offspring of inbred maternal plants had reduced spine production, compared to those of outbred plants, and caterpillars performed better on these plants. Both herbivory and inbreeding in the maternal generation altered volatile emissions of offspring. In general, maternal plant inbreeding dampened transgenerational effects of herbivory on offspring defensive traits and herbivore resistance. Taken together, this study demonstrates that inducible defenses in S. carolinense can persist across generations and that inbreeding compromises transgenerational resistance in S. carolinense.