Canine parvovirus (CPV) is a non-enveloped virus that replicates in the nucleus during infection. While it is known that it uses the cell?s microtubule network to traverse the cytoplasm, the route of nuclear entry is unclear. The nuclear pore complex was thought to be used by the virus due to the presence of nuclear localization sequences (NLS) in the VP1 protein. But recent studies in the minute virus of mice (MVM) have proposed that it induces breaks in the nuclear envelop before entering the nucleus via these breaks. In this study, Crandell Feline Kidney (CRFK) cells stably transfected with GFP-lamin A/C were micro-injected with CPV capsids whose distribution within the cells was then detected using fluorescent-labeled antibodies. The CPV capsids did not appear to induce breaks in the nuclear lamin structure of the cells that was observed for the MVM.