Understanding the role of defects and disorders based on a rigorous assessment of material properties is essential in the process of developing materials for the application. In this dissertation, I study the effect of structural distortions in organic radical polymers and chiral magnet composed of ferromagnetic heterostructure. In the first study, I demonstrate the high resistive nature of organic polymers with pendent stable radicals using the rigorous assessment of radical density and conductivity. I will also present the exponential decay of the conductivity of thin film conjugated polymer with increasing ratio of pendant radical, which is explained by the increasing disorders from steric hindrance that pendent radicals cause. In the second theme, I develop an operando current pulsing device for the Lorentz transmission electron microscope and examine the behavior of skyrmion in response to a stimulus. While the skyrmions are robustly created in the presence of structural defects, no sign of movement is observed due to the pinning effect. I will also explain the thermal origin of the creation and annihilation of skyrmions by the current pulse and use it to control the density of skyrmions and test its stability against magnetic field perturbation.