Micro X-Ray Fluorescence, Electron Microprobe Analysis, Raman Spectroscopy and Laser Ablation Inductively Coupled Plasma Mass Spectrometry were applied to identify the mineral hosts of five deleterious elements found in trace amounts within sphalerite ore in the historical Balmat Mining District. These deleterious elements include iron, copper, magnesium, mercury, and arsenic, which can potentially be mitigated through geometallurgical strategies such as ore sorting, real-time feedstock analysis, and roasting. The contents of secondary fluid inclusions within the sphalerite ore contained CO2, SO2, carbonate and tetrahedrite, which represent the composition of metamorphic fluids. Presence of these deleterious elements, antimony, and fluid inclusions within gray marble of Unit #7 may be used as exploration vectors.