These files contain data supporting all results reported in M Martin-Flores et al, EVJ-OA-23-200.R1 - Low-dose sugammadex reverses moderate rocuronium-induced neuromuscular block in horses. Fourteen adult horses undergoing different procedures were anesthetized with detomidine and isoflurane. All horses received NMB with rocuronium 0.3 mg/kg IV. Neuromuscular function was measured with acceleromyographic train-of-four (TOF) ratio. Recovery occurred spontaneously in five horses weighing [median (range)] 548 (413 – 594) kg and was enhanced with sugammadex 200 mg (total dose) in nine horses [433 (362 – 515)] kg. Recovery time from moderate NMB to a TOF ratio 1.0, and total duration of NMB were compared between groups. The dose of sugammadex was 0.46 (0.39 – 0.55) mg/kg. The recovery period lasted 21 (17 – 39) minutes for spontaneous and 4 (3 – 7) minutes for sugammadex. Total duration of NMB was 58 (41 – 70) minutes for spontaneous and 36 (21 – 43) for sugammadex (both p ≤ 0.003). There were no instances of recurarization.