Direct Analysis in Real-Time Mass Spectrometry (DART-MS) and Nuclear Magnetic Resonance (NMR) are two analytical techniques that are most used in structural chemistry and biology. DART-MS measures mass to charge ratio of different compounds in a solution. From the mass to charge ratio, the chemical formula of a substance can be deduced. NMR can determine the chemical structure of a purified substance. Thus, with the chemical information of molecules gathered by DART-MS and NMR, chemical structures of the molecules can be unambiguously determined. In this thesis, the chemical structure of an unknown compound was found by DART-MS and NMR. The unknown compound was dissolved in chloroform (CDCl3) solvent, and the exact mass of the unknown compound was measured by DART-MS. After the mass measurement, the solution was run in NMR for the 1H NMR and 2D NMR. With the data from DART-MS and NMR structure, the chemical structure of the compound was determined to be nuciferine.