Agricultural productivity relies on synthetic nitrogen fertilizers, yet half of that reactive nitrogen is lost to the environment (Wen et al., 2021). In today’s agriculture, farmers are facing two main challenges: increasing their yield production while reducing their environmental footprint. Nitrogen (N) is a macronutrient required to ensure optimal growth on corn fields. Insufficient nitrogen can lead to yield loss, while its excessive use can result in harmful environmental issues. To face the dilemma, farmers are experimenting with various techniques and new technologies to find an alternative to synthetic N fertilizers. One such alternative is Biological N Fixation that involves genetically modified bacteria capable of fixing nitrogen from the air. This research project aims to support farmers in their experimentation with biologicals by measuring the presence and levels of PivotBio in the soil, a genetically modified bacteria, in their on-farm trials.