Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change.