Microbiology Monographs, Papers and Research
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This is a collection of Papers, Research and Monographs in the area of Microbiology .
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Item Data from: A mutation in RNA polymerase that imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolismPatel, Yesha; Helmann, John D. (2024)These files contain RNAseq dataset reported in "A mutation in RNA polymerase that imparts resistance to β-lactams by preventing dysregulation of amino acid and nucleotide metabolism" by Patel et al. In this study, we compared two RNAP mutations with opposite effects on β-lactam resistance in Bacillus subtilis. A substitution in β’ (rpoC G1122D) increased resistance, whereas one in β (rpoB H482Y) increased sensitivity. RNA sequencing uncovered a decrease in branched-chain amino acid (BCAA), methionine, and pyrimidine pathways specifically in the β-lactam-resistant strain. These same pathways were up-regulated by cefuroxime (CEF), a model β-lactam in wild-type, but not in the CEF-resistant rpoC mutant. Genetic derepression of BCAA or pyrimidine synthesis also increased CEF sensitivity. Thus, β-lactams can alter the transcriptome and thereby contribute to drug toxicity, and RNAP mutations can impede these deleterious changes. These findings provide a vivid example of how RNAP mutations, which commonly arise in response to diverse selection conditions, can alter the transcriptome resulting in a rewiring of cellular metabolism to enhance fitness.Item Data used in analyses associated with: "Salinity-aided selection of progressive onset denitrifiers as a means of providing nitrite for anammox"Shapleigh, James P; Roco, Armanda (2018)This is the protein sequence dataset used for determining reads derived from denitrification genes in Illumina read samples. The protein types in the dataset are the active site containing subunits from the following proteins: Nap – periplasmic nitrate reductase; Nar – membrane associated nitrate reductase; NirK – copper containing nitrite reductase; NirS- heme-containing nitrite reductase; cNor – cytochrome c oxidizing nitrite reductase; qNor – quinol oxidizing nitric oxide reductase and Nos – nitrous oxide reductase. Sequences were initially obtained from the data available from the Integrated Microbial Genomes (IMG) system (https://img.jgi.doe.gov/cgi-bin/m/main.cgi). Related proteins were then aligned and proteins predicted to not be related or that were significantly shorter in length than group average were removed.Item The gut microbiome of TLR5-/- mice displays increased motility, which can be inhibited by anti-flagellin antibodiesCullender, Tyler (2013-02-15)TLR5-/- mice have an innate immune deficiency in recognizing bacterial flagellin, which results in increased flagellin expression by the microbiota and decreased anti-flagellin antibodies produced by the host. Here we show that the gut microbiome of TLR5-/- mice displays increased motility compared to wild-type controls (WT). The addition of antibodies with specificity to the flagellin protein of Roseburia hominis (Fla2) and Escherichia coli (FliC) decreased motility in bacteria from both mouse types within one hour.Item Anti-flagellin antibodies inhibit motility in Roseburia intestinalis and Clostridium ramosumCullender, Tyler (2013-02-11)Here we show that antibodies with specificity to the flagellin protein of Roseburia hominis (Fla2) and Escherichia coli (FliC) are capable of inhibiting motility of cultured Roseburia intestinalis and Clostridium ramosum within 30 minutes of application. Anti-mouse antibodies are included as a specificity control.