ItemData used in analyses associated with: "Salinity-aided selection of progressive onset denitrifiers as a means of providing nitrite for anammox"Shapleigh, James P; Roco, Armanda (2018)This is the protein sequence dataset used for determining reads derived from denitrification genes in Illumina read samples. The protein types in the dataset are the active site containing subunits from the following proteins: Nap – periplasmic nitrate reductase; Nar – membrane associated nitrate reductase; NirK – copper containing nitrite reductase; NirS- heme-containing nitrite reductase; cNor – cytochrome c oxidizing nitrite reductase; qNor – quinol oxidizing nitric oxide reductase and Nos – nitrous oxide reductase. Sequences were initially obtained from the data available from the Integrated Microbial Genomes (IMG) system (https://img.jgi.doe.gov/cgi-bin/m/main.cgi). Related proteins were then aligned and proteins predicted to not be related or that were significantly shorter in length than group average were removed. ItemThe gut microbiome of TLR5-/- mice displays increased motility, which can be inhibited by anti-flagellin antibodiesCullender, Tyler (2013-02-15)TLR5-/- mice have an innate immune deficiency in recognizing bacterial flagellin, which results in increased flagellin expression by the microbiota and decreased anti-flagellin antibodies produced by the host. Here we show that the gut microbiome of TLR5-/- mice displays increased motility compared to wild-type controls (WT). The addition of antibodies with specificity to the flagellin protein of Roseburia hominis (Fla2) and Escherichia coli (FliC) decreased motility in bacteria from both mouse types within one hour. ItemAnti-flagellin antibodies inhibit motility in Roseburia intestinalis and Clostridium ramosumCullender, Tyler (2013-02-11)Here we show that antibodies with specificity to the flagellin protein of Roseburia hominis (Fla2) and Escherichia coli (FliC) are capable of inhibiting motility of cultured Roseburia intestinalis and Clostridium ramosum within 30 minutes of application. Anti-mouse antibodies are included as a specificity control.