Adjustment Of Surface Chemical And Physical Properties With Functional Polymers To Control Cell Adhesion
| dc.contributor.author | Zhou, Zhaoli | en_US |
| dc.contributor.chair | Ober, Christopher Kemper | en_US |
| dc.contributor.committeeMember | Fischbach, Claudia | en_US |
| dc.contributor.committeeMember | Baird, Barbara Ann | en_US |
| dc.date.accessioned | 2013-09-05T15:26:37Z | |
| dc.date.available | 2018-01-29T07:00:42Z | |
| dc.date.issued | 2013-01-28 | en_US |
| dc.description.abstract | Cell-surface interaction is crucial in many cellular functions such as movement, growth, differentiation, proliferation and survival. In the present work, we have developed several strategies to design and prepare synthetic polymeric materials with selected cues to control cell attachment. To promote neuronal cell adhesion on the surfaces, biocompatible, non-adhesive PEG-based materials were modified with neurotransmitter acetylcholine functionalities to produce hydrogels with a range of porous structures, swollen states, and mechanical strengths. Mice hippocampal cells cultured on the hydrogels showed differences in number, length of processes and exhibited different survival rates, thereby highlighting the importance of chemical composition and structure in biomaterials. Similar strategies were used to prepare polymer brushes to assess how topographical cues influence neuronal cell behaviors. The brushes were prepared using the "grown from" method through surface-initiated atom transfer radical polymerization (SI-ATRP) reactions and further patterned via UV photolithography. Protein absorption tests and hippocampal neuronal cell culture of the brush patterns showed that both protein and neuronal cells can adhere to the patterns and therefore can be guided by the patterns at certain length scales. We also prepared functional polymers to discourage attachment of undesirable cells on the surfaces. For example, we synthesized PEG-perfluorinated alkyl amphiphilic surfactants to modify polystyrene-block-poly(ethylene-ran-butylene)-block-polyisoprene (SEBI or K3) triblock copolymers for marine antifouling/fouling release surface coatings. Initial results showed that the polymer coated surfaces can facilitate removal of Ulva sporelings on the surfaces. In addition, we prepared both bioactive and dual functional biopassive/bioactive antimicrobial coatings based on SEBI polymers. Incubating the polymer coated surfaces with gram-positive bacteria (S. aureus), gram-negative bacteria (E. coli) and marine bacteria (C. marina) species demonstrated that, unlike biopassive surfaces, the dual functionality polymer coated surfaces can significantly reduce both live and dead cells, without killing the cells in the culture media. The knowledge gained from those studies offers opportunities for further modification and potential applications of those types of polymers in the future. | en_US |
| dc.identifier.other | bibid: 8267709 | |
| dc.identifier.uri | https://hdl.handle.net/1813/33970 | |
| dc.language.iso | en_US | en_US |
| dc.subject | Hydrogels | en_US |
| dc.subject | polymer brushes | en_US |
| dc.subject | antifouling | en_US |
| dc.subject | antimicrobial | en_US |
| dc.subject | biomaterials | en_US |
| dc.subject | amphiphilic polymers | en_US |
| dc.title | Adjustment Of Surface Chemical And Physical Properties With Functional Polymers To Control Cell Adhesion | en_US |
| dc.type | dissertation or thesis | en_US |
| thesis.degree.discipline | Chemistry and Chemical Biology | |
| thesis.degree.grantor | Cornell University | en_US |
| thesis.degree.level | Doctor of Philosophy | |
| thesis.degree.name | Ph. D., Chemistry and Chemical Biology |
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