Zhang, Wenyao2024-04-052023-08Zhang_cornellgrad_0058F_13947http://dissertations.umi.com/cornellgrad:13947https://hdl.handle.net/1813/114821220 pagesThe study of bacterial stress response systems is an important area of research with wide-ranging implications, from understanding the basic bacterial physiology to developing new antimicrobial treatments. This dissertation presents my recent efforts to understand how Gram-negative bacteria adapt to environmental stimuli using live cell fluorescence imaging, genetic engineering, bulk biochemical/cellular assays, and microfluidic mechanical manipulations. Employing these methods, I, in collaboration with others, investigated three types of stress response systems associated with the Gram-negative bacteria cell envelope: multidrug efflux pumps (MEPs), two-components signal transduction systems (TCSs), and metal homeostasis.enBiomechanicsCell-to-cell communicationMultidrug efflux pumpsProtein interactionsSuper-resolution imagingTwo-component systemsSingle-molecule, single-cell imaging of stress response systems in Gram-negative bacteriadissertation or thesishttps://doi.org/10.7298/yq70-mf23