2026-09-19T00:08:07Zhttps://ecommons.cornell.edu/server/oai/requestoai:ecommons.cornell.edu:1813/1158632026-05-15T19:41:37Zcom_1813_35col_1813_47
Sheets, Jocelyn
Selvaraj, Vimal
Ren, Yi
Cheong, Soon Hon
2024-11-05T19:42:56Z
2024-11-05T19:42:56Z
2024-05
ProQuest Submission ID: 12087
ProQuest Publication ID: 31242656
https://hdl.handle.net/1813/115863
https://doi.org/10.7298/qf31-w285
16575616
79 pages
Steroid hormones are critical for physiological regulation and reproduction. Starting with cholesterol, steroids are initially transformed into pregnenolone by the enzyme P450scc (CYP11A1). Pregnenolone is then converted into various steroid intermediates by 3-β hydroxysteroid dehydrogenase/ Δ5-Δ4 isomerase (HSD3B), a process crucial for producing key hormones like progesterone and testosterone— deficiencies in HSD3B lead to disorders such as congenital adrenal hyperplasia and disrupted sexual development. There are several isoforms of HSD3B in mice and humans that show distinct tissue expressions in organs like the adrenal cortex and gonads. However, its subcellular localization that provides the cellular site of enzyme activity remains confusing and has been a topic of debate. Our study explores the subcellular localization of HSD3B using an approach for precise immunofluorescence, providing clarity on its site of action, and enhancing understanding of its biosynthetic function in steroidogenesis.
en
STUDIES ON THE SUBCELLULAR LOCALIZATION OF 3-BETA- HYDROXYSTEROID DEHYDROGENASE IN STEROIDOGENIC CELLS
dissertation or thesis
https://newcatalog.library.cornell.edu/catalog/16575616
application/pdf
Animal Science
Cornell University
Master of Science
M.S., Animal Science
https://hdl.handle.net/1813/59810.2
Publication
Cornell Theses and Dissertations
Sheets, Jocelyn
5893a6ea-7af3-41d7-abc6-04bcd26ab5df
open.access
open.access
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<Abstract>Steroid hormones are critical for physiological regulation and reproduction. Starting with cholesterol, steroids are initially transformed into pregnenolone by the enzyme P450scc (CYP11A1). Pregnenolone is then converted into various steroid intermediates by 3-β hydroxysteroid dehydrogenase/ Δ5-Δ4 isomerase (HSD3B), a process crucial for producing key hormones like progesterone and testosterone— deficiencies in HSD3B lead to disorders such as congenital adrenal hyperplasia and disrupted sexual development. There are several isoforms of HSD3B in mice and humans that show distinct tissue expressions in organs like the adrenal cortex and gonads. However, its subcellular localization that provides the cellular site of enzyme activity remains confusing and has been a topic of debate. Our study explores the subcellular localization of HSD3B using an approach for precise immunofluorescence, providing clarity on its site of action, and enhancing understanding of its biosynthetic function in steroidogenesis.</Abstract>
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