2026-09-20T12:24:49Zhttps://ecommons.cornell.edu/server/oai/requestoai:ecommons.cornell.edu:1813/1145062026-05-15T19:40:29Zcom_1813_35col_1813_47
Meng, Wei
Gan, Susheng
Griffiths, Phillip
2024-04-05T18:36:42Z
2023-08
ProQuest Submission ID: 11728
ProQuest Publication ID: 30485200
https://hdl.handle.net/1813/114506
https://doi.org/10.7298/5c29-xh92
16219197
142 pages
Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change.
en
broccoli
abiotic stress
senescence
NAP
CRISPR/Cas9
resilience
Breeding broccoli resilient to multi-abiotic stresses using genome editing
dissertation or thesis
https://newcatalog.library.cornell.edu/catalog/16219197
application/pdf
Horticulture
Cornell University
Master of Science
M.S., Horticulture
https://hdl.handle.net/1813/59810.2
Publication
Cornell Theses and Dissertations
Meng, Wei
5893a6ea-7af3-41d7-abc6-04bcd26ab5df
open.access
open.access
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<Abstract>Broccoli cultivars are sensitive to environmental stresses. Dramatic fluctuation in temperature and other weather conditions caused by climate change has negatively affected the growth and development of broccoli and reduced broccoli productivity and quality. Plant senescence is one of the most important consequences of all adverse environmental conditions or abiotic stresses. NAC-LIKE, ACTIVATED BY AP3/PI (NAP), a NAC (NAM, ATAF1/2, and CUC2) family transcription factor, is a key regulator of abiotic stress-induced senescence. Knocking BoNAP out via CRISPR/Cas9 genome editing in broccoli (Brassica oleracea var. italica) renders the broccoli lines more resistant to multiple abiotic stresses including drought, salt, extreme temperatures, strong UV radiation, and darkness phenotypically and physiologically. The research provides a novel strategy for breeding broccoli germplasm resilient to climate change.</Abstract>
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