Arginine and proline metabolism is associated with older adult skeletal muscle mass and altered by inflammation in primary human muscle cells

Gupta, Diwakar

Arginine and proline metabolism is associated with older adult skeletal muscle mass and altered by inflammation in primary human muscle cells

Abstract

Age-related loss of skeletal muscle mass (sarcopenia) adversely affects muscle tissue-specific and whole-body homeostasis, suggesting an undefined relationship between muscle mass and whole-body circulation that underlies the etiology of sarcopenia. To investigate, 344 metabolites and analytes were measured in serum from 19 older adults. Arginine and proline metabolism was the pathway most closely associated with skeletal muscle index (SMI), a measure of skeletal muscle mass. Subsequently, gene expression of related transport and regulatory enzymes was measured. Younger and older adult muscle tissue had similar inflammatory signaling and gene expression of CAT-1 and CAT-2, the cationic amino acid transporters that allow arginine into muscle. However, the proinflammatory cytokine TNF-α increased CAT-2 gene expression in differentiated human primary skeletal muscle cells. TNF-α also altered the expression of arginine metabolic genes regulating polyamine synthesis and proline production. Changes in arginine availability and metabolism may underlie the relationship between inflammation and skeletal muscle mass.

Date Issued

2017-08-30

Keywords

Nutrition; metabolomics; arginine metabolism; inflammation susceptibility; myoblasts; sarcopenia

Committee Chair

Thalacker-Mercer, Anna E.

Committee Member

Libert, Sergiy
Brenna, James Thomas
Gu, Zhenglong

Degree Discipline

Nutrition

Degree Name

M.S., Nutrition

Degree Level

Master of Science

Types

dissertation or thesis