During the Apollo missions, astronauts reported nasal and eye irritation due to lunar dust exposure upon return from lunar extra-vehicular activities. Lunar dust includes pneumatic size particles in the range of<O.l um to ]Oum, composed of minerals like iron and silica and adhesive/abrasive properties. Studies have proven lunar dust toxicity on pulmonary tissue. However, a recent study on rabbit eyes showed minimal irritancy of the ocular surface from respirable and coarser unground lunar dust particles. To date, no studies have been conducted to assess the effects of lunar dust toxicity at the molecular level. In a tissue sharing effort derived from a parent lunar dust nose-only inhalation study in which rats were exposed to lunar dust, we assessed gene profiles in cornea RN A collected from rats I and 7 days after the exposure. Microarray analysis was performed. An Ingenuity iReportTM was then generated for canonical pathway identification.