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IDENTIFYING CELL-TYPE SPECIFIC TRANSCRIPTION FACTORS REGULATING PAUSE FORMATION AND/OR PAUSE RELEASE

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2025-09-05
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Abstract

Gene transcription is vital for cellular homeostasis, development, disease, and stress. It is subject to regulatory control by specific transcription factors that modulate distinct steps of the transcription cycle. Transcription factors that act on distinct steps within the transcription cycle have yet to be fully characterized. Here, we show that K562 factors EGR1, MITF and GATA1 promote Pol II's transition to productive elongation. Our study revealed that perturbation of specific transcription factors (TFs) like TAL1 and ESRRA could result in subtle effects at gene bodies and pause sites. The outcomes of our results are crucial in enhancing our understanding of the mechanisms by which TFs work together to generate diverse transcription patterns during development and in response to various metabolic, hormonal, and environmental signals.

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135 pages

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2023-08

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Union Local

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Lis, John

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Danko, Charles
Pugh, Benjamin

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Genetics, Genomics and Development

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Ph. D., Genetics, Genomics and Development

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Doctor of Philosophy

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Government Document

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dissertation or thesis

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