The use of reverse line-blot hybridization for the detection of Trichostrongylid nematodes in small ruminants

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Abstract

Helminth infections are among the top causes of economic loss in the upkeep of small ruminants, and with anthelmintic resistance on the rise, it is vital that rapid and versatile diagnostic tests be developed to better inform farmers and veterinarians and to assist in their treatment decisions. In this study, reverse line-blot hybridization is explored for the genusspecific detection of three major abomasal parasites of sheep and goats: Haemonchus, Trichostrongylus, and Teladorsagia. Using previously developed genus-specific primers complementary to internal transcribed spacer 2 (ITS-2), this assay allows for the specific detection of H. contortus and the nonspecific detection of the remaining two abomasal parasites using a cross-hybridizing probe. Although additional research is needed to improve assay specificity, the technique explored here provides a potential high-throughput alternative for the detection of H. contortus, and shows promise for future applications in the detection of anthelmintic resistance-conferring single nucleotide polymorphisms (SNPs).

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2018-05
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Biological sciences honors program; parasitic nematodes; RLBH; small ruminants; Haemonchus; Trichostrongylus; Teladorsagia; diagnosis
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Biological Sciences
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B.A., Biological Sciences
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Bachelor of Arts
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Government Document
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dissertation or thesis
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