eCommons

 

Nucleic Acid-Protein Hybrid Materials

Other Titles

Author(s)

Abstract

DNA nanotechnology was used to interface with proteins through DNAprotein hybrid molecules for protein detection and protein intracellular delivery. EZZ protein, which can bind to almost all IgG antibodies, was selected to be conjugated to DNA linker. Different methods of DNA-protein conjugation were used to develop universal adapters between IgG antibodies and DNA. Using DNA nanobarcodes, which is based on the color ratios of two colors rather than using different colors, a library of IgG-based nanobarcodes were generated through a simple mixing step. The IgG-based nanobarcodes were used as a platform to simultaneously detect target proteins in different systems, including microbead-based, membrane-based, and tissue-based. Moreover, for the first time, a new concept of using DNA transfection reagents was introduced to deliver proteins intracellularly with great efficiency.

Journal / Series

Volume & Issue

Description

Sponsorship

Date Issued

2012-05-27

Publisher

Keywords

Nucleic Acids; Protein; Materials

Location

Effective Date

Expiration Date

Sector

Employer

Union

Union Local

NAICS

Number of Workers

Committee Chair

Luo, Dan

Committee Co-Chair

Committee Member

Lis, John T
March, John C

Degree Discipline

Agricultural and Biological Engineering

Degree Name

Ph. D., Agricultural and Biological Engineering

Degree Level

Doctor of Philosophy

Related Version

Related DOI

Related To

Related Part

Based on Related Item

Has Other Format(s)

Part of Related Item

Related To

Related Publication(s)

Link(s) to Related Publication(s)

References

Link(s) to Reference(s)

Previously Published As

Government Document

ISBN

ISMN

ISSN

Other Identifiers

Rights

Rights URI

Types

dissertation or thesis

Accessibility Feature

Accessibility Hazard

Accessibility Summary

Link(s) to Catalog Record