Simulating the Limits of Multiphoton Microscopy

Other Titles
Abstract
Multiphoton microscopy is an important tool for high-resolution imaging of turbid media. A fundamental limit of the technique is the excitation produced outside of the focal region. The ratio of the signal at the focus to the background--the signal-to-background ratio, or SBR--is one important quantity that determines the limit of depth that can be imaged in a multiphoton experiment. While it is often believed that only unscattered light contributes to this background, scattered light is also a significant component. In this thesis a convolutional method of solving for scattered light will be used and adjusted for the multiphoton problem. Additionally, a novel Monte Carlo formulation is implemented on the GPU for this same purpose. The two techniques give similar results for produced excitations and SBR. Both demonstrate the primary importance of scattered light in the generation of out-of-focus excitation. Each method also shows the strong dependence of the SBR on a variety of illumination and optical properties.
Journal / Series
Volume & Issue
Description
100 pages
Sponsorship
Date Issued
2021-05
Publisher
Keywords
Microscopy; Monte Carlo; Multiphoton
Location
Effective Date
Expiration Date
Sector
Employer
Union
Union Local
NAICS
Number of Workers
Committee Chair
Xu, Chris
Committee Co-Chair
Committee Member
Schaffer, Chris
Degree Discipline
Applied Physics
Degree Name
M.S., Applied Physics
Degree Level
Master of Science
Related Version
Related DOI
Related To
Related Part
Based on Related Item
Has Other Format(s)
Part of Related Item
Related To
Related Publication(s)
Link(s) to Related Publication(s)
References
Link(s) to Reference(s)
Previously Published As
Government Document
ISBN
ISMN
ISSN
Other Identifiers
Rights
Rights URI
Types
dissertation or thesis
Accessibility Feature
Accessibility Hazard
Accessibility Summary
Link(s) to Catalog Record