Development Of Micro And Nanoparticle Substrates For Sers Detection Of Pesticides And Delivery Of Chemotherapeutic Drugs And Pilot Scale Process Development And Cgmp Production Of Cancer Testis Antigen Melan-A
Loading...
Files
No Access Until
Permanent Link(s)
Collections
Other Titles
Authors
Abstract
Detection or delivery of a diverse range of biomolecules in field portable devices is an important area of research in the fields of environmental pollution, homeland security, and medicine. The demand for hand-held and low cost detection in these fields has led to the development of novel analytical systems such as Surface Enhanced Raman Spectroscopy (SERS) and Molecularly Imprinted Polymers (MIP). SERS is rapidly emerging as a tool for biological assays, chemical sensing, and electrochemistry. Whereas, Molecularly Imprinted Polymers have been developed as systems for solid phase extraction, chemical separation, and controlled release. In this dissertation, several systems were developed using nanotechnology and polymer synthesi s to develop chemical sensors and a drug delivery system. A substrate for pesticide detection was developed by conjugating oligonucleotides specific for the pesticide malathion to the surface of a SERS substrate that detected the organophosphorus pesticide malathion down to the micromolar level. A SERS substrate was also developed by grafting a molecularly imprinted polymer of methacrylic acid and ethylene glycol dimethacrylate to surface of a gold coated silica microparticle that was able to capture and detect the pesticide thiabendazole. Molecularly ii imprinted polymers were investigated as a drug delivery system for the chemotherapeutic doxorubicin which showed strong imprinting and temperature sensitive controlled release of drug. Lastly, a cGMP pilot scale process was developed to produce gram levels of the cancer vaccine Melan-A for phase I clinical trials. iii
Journal / Series
Volume & Issue
Description
Sponsorship
Date Issued
2013-05-26
Publisher
Keywords
Surface Enhance Raman Spectroscopy; Molecularly Imprinted Polymers; Cancer Testes Antigens
Location
Effective Date
Expiration Date
Sector
Employer
Union
Union Local
NAICS
Number of Workers
Committee Chair
Batt, Carl A
Committee Co-Chair
Committee Member
Angert, Esther R.
Delisa, Matthew
Delisa, Matthew
Degree Discipline
Biomedical Engineering
Degree Name
Ph. D., Biomedical Engineering
Degree Level
Doctor of Philosophy
Related Version
Related DOI
Related To
Related Part
Based on Related Item
Has Other Format(s)
Part of Related Item
Related To
Related Publication(s)
Link(s) to Related Publication(s)
References
Link(s) to Reference(s)
Previously Published As
Government Document
ISBN
ISMN
ISSN
Other Identifiers
Rights
Rights URI
Types
dissertation or thesis