eCommons

 

INFLUENCE OF RED WINE GRAPE PHENOLIC EXTRACTS ON GLUCOSYLTRANSFERASE ACTIVITY, ACIDOGENICITY, AND BIOFILM FORMATION OF STREPTOCOCCUS MUTANS.

Other Titles

Abstract

Wine grapes are rich sources of potentially bioactive polyphenols. However, phenolic content is variable depending on grape variety, and may be modified during vinification. In this study, we investigated the influence of phenolic extracts from Vitis vinifera (Cabernet Franc and Pinot noir), V. Labrusca (Concord), and Vitis hybrids (Baco noir and Noiret) grapes on several virulence properties of Streptococcus mutans a well-known cariogenic bacterium. Grape phenolic extracts were prepared from whole fruit (WF) and fermented pomace (FP). The anthocyanin and flavan-3-ol content were variable depending on variety and type of extract (WF vs. FP). Nevertheless, all extracts inhibited the activity of GTF B between 60-80% at concentrations as low as 31.2 ug/ml; GTF B synthesizes insoluble glucans, which are critical for the formation, bulk and structural integrity of biofilms. Furthermore, the glycolytic pH-drop by S. mutans cells was inhibited by the extracts without affecting bacterial viability, an effect that can be attributed to partial inhibition of F-ATPase by the extracts (30-65% inhibition at 125 ug/ml). In general, phenolic extracts from V. vinifera were more effective than those from Vitis hybrids. The biological activity of FP was either as effective as or significantly better than WF extracts. The most effective extracts were examined for their ability to disrupt biofilm development by S. mutans using a saliva-coated hydroxyapatite biofilm model. Biofilms were treated for 1 minute twice daily with phenolic extracts from FP of Cabernet Franc and Baco noir. Biofilms treated with the extracts (at 1 mg/ml) resulted in significantly lower amounts of extracellular insoluble polysaccharides than those treated with vehicle control (P<0.01), which agrees well with the GTF B inhibition data. The reduction of insoluble polysaccharides production is highly relevant because they are the main components of the extracellular matrix of biofilms, and are associated with the pathogenesis of dental caries. Overall, the phenolic extracts, especially from pomace, are effective against specific virulence attributes of S. mutans and can disrupt the synthesis of extracellular polysaccharide matrix of biofilms. Fermented pomace could be a potential source for extraction of bioactive compounds for prevention or reduction of oral diseases, such as dental caries.

Journal / Series

Volume & Issue

Description

Sponsorship

This project was supported by the Field of Food Science and Technology SUNY fellowship program and the National Research Initiative Grant No. 2006-35200-16589 from the USDA Cooperative State Research, Education, and Extension Service Bioactive Food Components for Optimal Health (31.0) program. Travel grants were provided by the Western New York Institute of Food Technologists section and the Department of Food Science.

Date Issued

2008-10-06T14:23:22Z

Publisher

Keywords

grape, streptococcus mutans, polyphenols, glucosyltransferases, ATPase, biofilms

Location

Effective Date

Expiration Date

Sector

Employer

Union

Union Local

NAICS

Number of Workers

Committee Chair

Committee Co-Chair

Committee Member

Degree Discipline

Degree Name

Degree Level

Related Version

Related DOI

Related To

Related Part

Based on Related Item

Has Other Format(s)

Part of Related Item

Related To

Related Publication(s)

Link(s) to Related Publication(s)

References

Link(s) to Reference(s)

Previously Published As

Government Document

ISBN

ISMN

ISSN

Other Identifiers

Rights

Rights URI

Types

dissertation or thesis

Accessibility Feature

Accessibility Hazard

Accessibility Summary

Link(s) to Catalog Record