eCommons

 

Elucidating the Reactivity of Cytochrome P460: Maturation, Product Selectivity, and Substrate Scope

Access Restricted

Access to this document is restricted. Some items have been embargoed at the request of the author, but will be made publicly available after the "No Access Until" date.

During the embargo period, you may request access to the item by clicking the link to the restricted file(s) and completing the request form. If we have contact information for a Cornell author, we will contact the author and request permission to provide access. If we do not have contact information for a Cornell author, or the author denies or does not respond to our inquiry, we will not be able to provide access. For more information, review our policies for restricted content.

No Access Until

2024-09-05
Permanent Link(s)

Other Titles

Abstract

Ammonia oxidizing bacteria (AOB) have evolved a nitrogen-based metabolism in which they rely solely on the oxidation of ammonia as their energy for life. In contrast to carbon-based metabolisms, there is a large gap in our knowledge of nitrogen-based metabolisms. The primary energy producing step by AOB is performed by the enzyme hydroxylamine oxidoreductase, which oxidizes hydroxylamine (NH2OH) to nitric oxide (NO). Another enzyme expressed by AOB, cytochrome P460, can also oxidize NH2OH but produces nitrous oxide (N2O). The ability of these enzymes to oxidize NH2OH is attributed in part to their unique heme P460 cofactors, which feature additional cross-links to the porphyrin. While a proposed function of cytochrome P460 in vivo is the detoxification of NH2OH, this remains speculative. This thesis will address the reactivity of cytochrome P460 and put it into the context of AOB metabolism. Cytochrome P460 can catalyze a variety of reactions, both as the proenzyme and matured cross-linked enzyme. I will interrogate the mechanisms employed by cytochrome P460 using a combination of spectroscopy and mutagenesis. By doing so, I hope to expand our understanding of the function(s) of cytochrome P460, highlighting its ability to do chemistry that had not previously been considered. The results herein will have important implications for AOB metabolism.

Journal / Series

Volume & Issue

Description

247 pages

Sponsorship

Date Issued

2023-08

Publisher

Keywords

Bioinorganic; Cytochrome P460; Hydroxylamine Oxidation; Nitrification; Spectroscopy

Location

Effective Date

Expiration Date

Sector

Employer

Union

Union Local

NAICS

Number of Workers

Committee Chair

Lancaster, Kyle

Committee Co-Chair

Committee Member

Crane, Brian
Ando, Nozomi

Degree Discipline

Chemistry and Chemical Biology

Degree Name

Ph. D., Chemistry and Chemical Biology

Degree Level

Doctor of Philosophy

Related Version

Related DOI

Related To

Related Part

Based on Related Item

Has Other Format(s)

Part of Related Item

Related To

Related Publication(s)

Link(s) to Related Publication(s)

References

Link(s) to Reference(s)

Previously Published As

Government Document

ISBN

ISMN

ISSN

Other Identifiers

Rights

Rights URI

Types

dissertation or thesis

Accessibility Feature

Accessibility Hazard

Accessibility Summary

Link(s) to Catalog Record